Bionas Discovery 2500 system

 

The Bionas DiscoveryTM 2500 system is available with 2 or 6 parallel Biomodules and monitors physiological parameters of living cells and tissue slices in a label-free  and non-invasive assay. Cells are placed on a metabolic chip, the Bionas DiscoveryTM SC1000, and supplied with medium in a perfusion system.

 

 

 

The multiparametric sensor chip continuously measures:

  • oxygen consumption
  • extracellular acidification

Both parameters determine the acute rates of the cellular energy metabolism (bioenergetics) thus providing essential information on the cellular status and the changes thereof in response to external stimuli. 

  • cell impedance

The cell impedance detects alterations in the cellular adhesion/confluence and morphology.

Long-term studies and regeneration

A perfusion system allows for parameter recordings over several days and makes it possible for the first time:

  • to monitor cellular responses to drug exposure in long-term studies
  • to estimate recovery afterwards for the same cell population
  • to collect information on adaptation effects after repeated drug exposure

Bioenergetics + cell impedance

The parameter profiles consistently reflect drug induced responses of the acute cellular energy metabolism and corresponding alterations in cell morphology (cell-cell contacts, cell-matrix interactions, ligand binding to receptors).

 

Fig. 1:

  • AAP exposed primary human hepatocytes with remarkable changes in metabolic rates while cell impedance remains unaffected. After removal of AAP cells recovered completely.
 
 
 
 
 
 
  
 
 
 
 
Fig. 2:
  • Diclofenac caused responses in HepG2 cells showing non-linear changes in bioenergetics and cell impedance. These effects are irreversible.